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R&D Systems
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Image Search Results
Journal: Cellular & molecular medicine: open access
Article Title: Potential Mechanisms Underlying TGF-β-mediated Complement Activation in Lung Fibrosis
doi:
Figure Lengend Snippet: RNA interference suppresses local complement activation. BALF collected from Figure 1 were analyzed for C3a (A) and C5a (B) levels in the lung by ELISA. Values: Means ± SEM. (n=5-7 per group). One-way ANOVA, Newman-Keuls (A, B). Compared to bleomycin: ***: p<0.001; **: p<0.01; *: p<0.05. Results are representative of three independent experiments.
Article Snippet: These studies used recombinant human C3a and
Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay
Journal: Cellular & molecular medicine: open access
Article Title: Potential Mechanisms Underlying TGF-β-mediated Complement Activation in Lung Fibrosis
doi:
Figure Lengend Snippet: miRNA regulation in response to TGF-β, C3a and C5a for 24 h in normal primary human SAECs.
Article Snippet: These studies used recombinant human C3a and
Techniques:
Journal: Oncoimmunology
Article Title: Blocking C5aR signaling promotes the anti-tumor efficacy of PD-1/PD-L1 blockade
doi: 10.1080/2162402X.2017.1349587
Figure Lengend Snippet: Anti-PD-L1 treatment leads to activation of the complement system. MC38 tumor-bearing mice (∼100 mm3) were intraperitoneally injected with anti-PD-L1 antibodies. The tumors were removed at the indicated time points (n = 5–6 mice/group). (A) C3a and C5a levels in homogenates from tumors and spleens were quantified by ELISA. (B) C1q and C3b/iC3b/C3c staining of tumors and spleens were quantified by IHC. (C) C3b/iC3b/C3c levels of homogenates from tumors and spleens were quantified by ELISA. One-way ANOVA (A) and Multiple t-test (C) were used to evaluate statistical significance (*p < 0.05, **p < 0.01).
Article Snippet: Biotinylated Rat Anti-Mouse C3a (clone I87–419, BD, 2 μg/ml) was used for detection, and purified
Techniques: Activation Assay, Injection, Enzyme-linked Immunosorbent Assay, Staining